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MISSION LentiPlex Samlede shRNA Library Screening i pattedyrceller
JoVE Journal
Biology
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JoVE Journal Biology
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells

MISSION LentiPlex Samlede shRNA Library Screening i pattedyrceller

DOI:
10.3791/3305-v

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09:12 min

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December 21, 2011

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, , , ,
1Sigma-Aldrich

Chapters

  • 00:05Title
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

Summary

Automatic Translation

Automatic Translation

Her bruker vi et menneske LentiPlex samlet bibliotek og tradisjonelle sekvensering metoder for å identifisere genet mål å fremme celle overlevelse. Vi viser hvordan du setter opp og deconvolute en LentiPlex skjermen og validere resultatene.

Tags

MISSION LentiPlexPooled ShRNA LibraryScreeningMammalian CellsRNA InterferenceGene Expression RegulationKnockdownLoss Of Gene Function StudiesSmall Interfering RNAsSiRNAsShort-hairpin RNAsShRNAsPlasmidsLentiviral ParticlesLentiviral ShRNAsIntroduction Into Cell TypesLong Term Gene KnockdownSelectionHigh-throughput Loss Of Function ScreensLentiPlex Pooled ShRNA LibraryGenome-wide Lentiviral PoolTRC CollectionHuman Genes

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