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MISSION LentiPlex Samlede shRNA Library Screening i pattedyrceller
JoVE Journal
Biology
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JoVE Journal Biology
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
DOI:

09:12 min

December 21, 2011

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Chapters

  • 00:05Title
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

Summary

Automatic Translation

Her bruker vi et menneske LentiPlex samlet bibliotek og tradisjonelle sekvensering metoder for å identifisere genet mål å fremme celle overlevelse. Vi viser hvordan du setter opp og deconvolute en LentiPlex skjermen og validere resultatene.

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