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MISSION LentiPlex Pooled shRNA Bibliotek Screening i pattedyrceller
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
MISSION LentiPlex Pooled shRNA Library Screening in Mammalian Cells
DOI:

09:12 min

December 21, 2011

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Chapters

  • 00:05Title
  • 01:40Transduction and Treatment of Target Cells
  • 04:04Deconvolution from a Polyclonal Cell Population
  • 06:16Target Identification and Validation
  • 07:25Representative Screening Results Identifying Genes Involved in the Cytotoxicity of A549 Cells when Exposed to TRAIL and Paclitaxel
  • 08:51Conclusion

Summary

Automatic Translation

Her bruger vi et menneskeligt LentiPlex samlet bibliotek og traditionelle sekventering metoder til at identificere genet mål at fremme celle overlevelse. Vi viser hvordan du opsætter og deconvolute en LentiPlex skærm og validere resultaterne.

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