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JoVE Journal
Medicine

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Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence
 

Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence

Article DOI: 10.3791/3334 07:55 min
October 25th, 2011

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Summary October 25th, 2011

Here we describe a method to quantify molecular heterogeneity in histological sections of tumor material using quantitative immunofluorescence, image analysis, and a statistical measure of heterogeneity. The method is intended for use in clinical biomarker development and analysis.

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