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Bioengineering
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旋转的人类细胞培养的细胞培养系统:作为一种模式的滋养细胞
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Rotating Cell Culture Systems for Human Cell Culture: Human Trophoblast Cells as a Model
Please note that all translations are automatically generated.
Click here for the English version.
旋转的人类细胞培养的细胞培养系统:作为一种模式的滋养细胞
DOI:
10.3791/3367-v
•
06:54 min
•
January 18, 2012
•
Kevin J. Zwezdaryk*
1
,
Jessica A. Warner*
1,2
,
Heather L. Machado
,
Cindy A. Morris
,
Kerstin Höner zu Bentrup
1
Department of Microbiology and Immunology
,
Tulane University Medical School
,
2
Physician/Scientist Program
,
Tulane University Medical School
,
3
Department of Molecular and Cellular Biology
,
Baylor College of Medicine
Chapters
00:05
Title
01:04
EVT Cells and Collagen Bead Incubation
02:13
Loading the Rotating Cell Culture System (RCCS)
03:41
Changing the Media
04:37
Collecting Propagated Cells
05:39
SGHPL-4 Trophoblast Cells in a 3D Milieu
06:30
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
传统的,二维细胞培养技术,往往导致与分化标志物,细胞因子和生长因子的改变的特点。旋转细胞培养系统的三维细胞培养(农村信用社)重新建立许多因素的表达,绒毛外滋养细胞系。
Tags
Rotating Cell Culture Systems
Human Cell Culture
Human Trophoblast Cells
Model
Primary Cultures
Explant Cultures
Trophoblast Cell Lines
Invasion Of Uterine Wall
Remodeling Of Uterine Spiral Arteries
Extravillous Trophoblast Cells
Successful Establishment Of Pregnancy
In Vitro Cell Culture Models
Rotating Cell Culture System (RCCS)
Morphological Properties
Phenotypic Properties
Functional Properties
EVT-like Cell Lines
Differentiation Of EVTs
Gene Expression
Matrix Metalloproteinases (MMPs)
Trophoblast Differentiation
Saint Georges Hospital Placental Cell Line-4 (SGHPL-4)
RCCS Culture Vessel
Three-dimensional Environment
Article
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