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RNAi screening for at identificere Postembryonic fænotyper i C. elegans</em
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JoVE Journal Biology
RNAi Screening to Identify Postembryonic Phenotypes in C. elegans

RNAi screening for at identificere Postembryonic fænotyper i C. elegans</em

DOI:
10.3791/3442-v

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09:40 min

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February 13, 2012

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,
1Department of Molecular and Cellular Medicine,Texas A&M University System Health Science Center

Chapters

  • 00:05Title
  • 01:24Preparation of Bacteria with Library Clones
  • 03:14Preparation of Nematodes
  • 06:51Observation of Nematodes
  • 08:55Conclusion

Summary

Automatic Translation

Automatic Translation

Beskriver vi en sensibiliseret metode til at identificere postembryonic regulatorer af proteinekspression og lokalisering i<em> C. elegans</em> Ved hjælp af en RNAi-baseret genomisk skærm og en integreret transgen som udtrykker et funktionelt, fluorescens mærket protein.

Tags

RNAi ScreeningC. ElegansPostembryonic PhenotypesModel SystemGenesGene PathwaysFunctional CharacterizationProtocolRNAi ScreenPhenotype AssayFluorescent ProductTransgeneGenomic RNAi LibraryCDNA InsertsRNAi-sensitive BackgroundHatched Animals

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