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Biology
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脂筏的测定分区在活细胞中的荧光标记探针的荧光相关光谱(FCS)
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JoVE Journal
Biology
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
Please note that all translations are automatically generated.
Click here for the English version.
脂筏的测定分区在活细胞中的荧光标记探针的荧光相关光谱(FCS)
DOI:
10.3791/3513-v
•
10:59 min
•
April 06, 2012
•
Catherine Marquer
,
Sandrine Lévêque-Fort
3
,
Marie-Claude Potier
1
Centre de Recherche de l’Institut du Cerveau et de la Moelle Épinière
,
Hôpital de la Pitié-Salpêtrière
,
2
Institut des Sciences Moléculaires d’Orsay
,
Université Paris-Sud
,
3
Centre de Photonique Biomédicale du Centre Laser
,
Université Paris-Sud
Chapters
00:05
Title
01:48
Calibration of the FCS Setup
03:45
FCS Data Analysis
05:50
Staining of Living Cells with Lipid-rafts Marker
06:51
FCS Data Acquisition on Living Cells
08:07
Representative FCS Analysis
10:18
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
描述一种技术来探测荧光蛋白在活细胞的细胞膜脂筏分区。它发生在位于内部或外部的脂筏蛋白的扩散时间的悬殊优势。收购可以进行动态控制条件或吸毒后。
Tags
Lipid Raft Partitioning
Fluorescently-tagged Probes
Living Cells
Fluorescence Correlation Spectroscopy
Cell Membranes
Microdomains
Cholesterol
Sphingolipids
Signalling
Trafficking
Diseases
Controversy
Lipid Raft Size
Microscopy Resolution Limit
Direct Imaging
Techniques
Detergent Resistant Membranes
Co-patching With Antibodies
Artefacts
Sensitive Analysis
Plasma Membrane
Article
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