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脂筏的测定分区在活细胞中的荧光标记探针的荧光相关光谱(FCS)
JoVE Journal
Biology
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JoVE Journal Biology
Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
DOI:

10:59 min

April 06, 2012

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Chapters

  • 00:05Title
  • 01:48Calibration of the FCS Setup
  • 03:45FCS Data Analysis
  • 05:50Staining of Living Cells with Lipid-rafts Marker
  • 06:51FCS Data Acquisition on Living Cells
  • 08:07Representative FCS Analysis
  • 10:18Conclusion

Summary

Automatic Translation

描述一种技术来探测荧光蛋白在活细胞的细胞膜脂筏分区。它发生在位于内部或外部的脂筏蛋白的扩散时间的悬殊优势。收购可以进行动态控制条件或吸毒后。

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