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Chapters
- 00:05Title
- 01:35Sample Preparation
- 02:42Isoelectric Focusing
- 04:17SDS Polyacrylamide Gel Electrophoresis
- 05:38In-gel Tryptic Digestion
- 07:38Results: Skeletal Muscle Protein
- 09:08Conclusion
En straight-forward sæt af metoder til at isolere og bestemme identiteten af de hyppigst forekommende proteiner udtrykt i skeletmuskulatur. Ca. 800 spots skimtes på en to-dimensional gel fra 10 mg muskler, hvilket giver mulighed for bestemmelse af kønsspecifikke protein udtryk. Disse metoder vil give tilsvarende resultater i de fleste væv.
Tags
Skeletal MuscleGender DimorphismProteomicsContractile ProteinsHypertrophyAtrophyRemodelingAdaptations To StressorsEccentric ContractionDamage To MuscleRepairDegradationResynthesisGlobal ProteomeExerciseProtein ExpressionDifferential Protein ExpressionAdaptations To Damage And RepairMale MuscleFemale MuscleTwo-dimensional Gel ElectrophoresisHigh Resolution Digital Imaging
