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改良酵母双杂交系统,以确定蛋白与生长因子Progranulin的相互作用
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Biology
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JoVE Journal
Biology
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin
Please note that all translations are automatically generated.
Click here for the English version.
改良酵母双杂交系统,以确定蛋白与生长因子Progranulin的相互作用
DOI:
10.3791/3562-v
•
07:56 min
•
January 17, 2012
•
Qing-Yun Tian
,
Yun-Peng Zhao
,
Chuan-ju Liu
2
1
Department of Orthopaedic Surgery
,
NYU Hospital for Joint Diseases
,
2
Department of Cell Biology
,
New York University School of Medicine
Chapters
00:05
Title
01:07
Small Scale Transformation of Bait Plasmid
02:12
Bait Validation
03:09
cDNA Library Screening
05:06
X-gal Assay
05:59
Retransformation in the MY2H Assay
06:47
Isolation of Progranulin-Interacting Proteins
07:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们已经修改了传统的酵母双杂交筛选,确定蛋白质相互作用的一种有效的遗传工具。此修改的过程明显缩短,减少了工作量,最重要的是,降低了误报数量。此外,这种方法是重复的,可靠的。
Tags
Modified Yeast-two-hybrid System
Proteins
Interacting
Growth Factor
Progranulin
PGRN
Granulin Epithelin Precursor
Autocrine Growth Factor
Physiologic Processes
Disease Processes
Inflammation
Host Defense
Neurotrophic Factor
PGRN Gene
Frontotemporal Dementia
Cartilage Development And Degradation
Binding Receptor(s)
Modified Yeast Two-hybrid Approach
GAL4 Based 2-hybrid System
False Positive Clones
Reproducible And Reliable System
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