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Modificeret Gær-Two-Hybrid system til at identificere Proteiner Interaktion med Growth Factor Progranulin
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JoVE Journal Biology
Modified Yeast-Two-Hybrid System to Identify Proteins Interacting with the Growth Factor Progranulin

Modificeret Gær-Two-Hybrid system til at identificere Proteiner Interaktion med Growth Factor Progranulin

DOI:
10.3791/3562-v

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07:56 min

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January 17, 2012

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, ,
1Department of Orthopaedic Surgery,NYU Hospital for Joint Diseases, 2Department of Cell Biology,New York University School of Medicine

Chapters

  • 00:05Title
  • 01:07Small Scale Transformation of Bait Plasmid
  • 02:12Bait Validation
  • 03:09cDNA Library Screening
  • 05:06X-gal Assay
  • 05:59Retransformation in the MY2H Assay
  • 06:47Isolation of Progranulin-Interacting Proteins
  • 07:32Conclusion

Summary

Automatic Translation

Automatic Translation

Vi har ændret det konventionelle gær to-hybrid screening, et effektivt genetisk redskab til at identificere protein interaktion. Denne ændring markant forkorter processen, reducerer arbejdsbyrden, og vigtigst, reducerer antallet af falske positiver. Hertil kommer, at denne tilgang reproducerbar og pålidelig.

Tags

Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System

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