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Chapters
- 00:05Title
- 01:07Small Scale Transformation of Bait Plasmid
- 02:12Bait Validation
- 03:09cDNA Library Screening
- 05:06X-gal Assay
- 05:59Retransformation in the MY2H Assay
- 06:47Isolation of Progranulin-Interacting Proteins
- 07:32Conclusion
Vi har ändrat den konventionella jäst två-hybrid screening, ett effektivt genetiska verktyg för att identifiera protein interaktion. Denna ändring förkortar markant processen, minskar arbetsbördan, och viktigast, minskar antalet falska positiva. Dessutom är denna metod reproducerbar och tillförlitlig.
Tags
Modified Yeast-two-hybrid SystemProteinsInteractingGrowth FactorProgranulinPGRNGranulin Epithelin PrecursorAutocrine Growth FactorPhysiologic ProcessesDisease ProcessesInflammationHost DefenseNeurotrophic FactorPGRN GeneFrontotemporal DementiaCartilage Development And DegradationBinding Receptor(s)Modified Yeast Two-hybrid ApproachGAL4 Based 2-hybrid SystemFalse Positive ClonesReproducible And Reliable System
