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增强细胞凋亡和自噬的诱导合成一种新型的C-1 7-deoxypancratistatin的类似物在人类乳癌细胞和神经母细胞瘤与他莫昔芬
JoVE Journal
Medicine
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JoVE Journal Medicine
Enhancement of Apoptotic and Autophagic Induction by a Novel Synthetic C-1 Analogue of 7-deoxypancratistatin in Human Breast Adenocarcinoma and Neuroblastoma Cells with Tamoxifen
DOI:

19:44 min

May 30, 2012

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Chapters

  • 00:05Title
  • 02:49Time-lapse Microscopy
  • 04:34Water Soluble Tetrazolium Salt (WST-1) Assay for Cell Viability
  • 07:05Mitochondrial Isolation
  • 08:34Amplex Red Assay for ROS Generation
  • 09:30Results: Selective Induction of Apoptosis in MCF7 and SH-SY5Y Cells by JCTH-4 and Enhancement of Activity by TAM
  • 18:29Conclusion

Summary

Automatic Translation

我们已合成了新型防癌抗癌作为本地pancratistatin活动相媲美的pancratistatin模拟;有趣的是,与他莫昔芬治疗组合的产生在细胞凋亡和自噬的诱导线粒体与非癌成纤维细胞的影响最小的目标大幅提高。因此,与他莫昔芬结合JCTH-4可以提供一个安全的抗癌疗法。

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