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リジン脱メチル化酵素活性の定量測定のためのMassSQUIRMのアプリケーション
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JoVE Journal
Biology
Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
Please note that all translations are automatically generated.
Click here for the English version.
リジン脱メチル化酵素活性の定量測定のためのMassSQUIRMのアプリケーション
DOI:
10.3791/3604-v
•
07:02 min
•
March 11, 2012
•
Lauren P. Blair
,
Nathan L. Avaritt
,
Alan J. Tackett
1
Department of Biochemistry and Molecular Biology
,
University of Arkansas for Medical Sciences
Chapters
00:05
Title
01:34
LSD1 Demethylation Assay
03:16
Reductive Methylation
04:09
MALDI Mass Spectrometry
04:59
Data Analysis
05:32
MassSQUIRM
06:31
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我々は、リジンメチル化の変化を定量化するためにMALDI質量分析法と還元的メチル化化学を使用するための手法を提案する。
Tags
MassSQUIRM
Quantitative Measurements
Lysine Demethylase Activity
Epigenetic Regulators
Small Molecule Studies
Drug Development
Histones
Proteins
Demethylase Assays
Qualitative Assays
Lysine Methylation States
Mass Spectrometry
Differential Ionization
Comprehensive Analysis
MassSQUIRM Method
Isotopic Reductive Methylation
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