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Biology

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Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
 

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Article DOI: 10.3791/52203
November 29th, 2014

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Summary November 29th, 2014

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

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