Journal
/
Biology
/
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

Specificity Analysis of Protein Lysine Methyltransferases Using SPOT Peptide Arrays

DOI:
10.3791/52203-v

•

08:48 min

•

November 29, 2014

•

, , ,
1Institute of Biochemistry,Stuttgart University

Chapters

  • 00:05Title
  • 01:54Preparation of Peptide Arrays
  • 04:48Peptide Array Methylation
  • 06:18Results: Characterization of Methylated Peptide Arrays
  • 07:38Conclusion

Summary

Automatic Translation

Automatic Translation

Peptide arrays synthesized by the SPOT method can be used to analyze the substrate specificity of Protein lysine methyltransferases (PKMTs) and to define the substrate spectrum of PKMTs to understand their biological role. This protocol describes how to synthesize peptide arrays, methylate them with PKMTs, and analyze the results.

Tags

Keywords: Protein Lysine MethyltransferasesLysine MethylationPost-translation ModificationCell DevelopmentDiseaseSubstrate SpecificityPeptide ArraySPOT Peptide ArraysNSD1H4K44H1.5K168H3K36

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52203?article_type=v

Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
check_url/52203?article_type=v

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery
check_url/52203?article_type=v

Mutagenesis and Functional Analysis of Ion Channels Heterologously Expressed in Mammalian Cells
check_url/52203?article_type=v

Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR (qPCR) Arrays
check_url/52203?article_type=v

Quantification of Proteins Using Peptide Immunoaffinity Enrichment Coupled with Mass Spectrometry
check_url/52203?article_type=v

Application of MassSQUIRM for Quantitative Measurements of Lysine Demethylase Activity
check_url/52203?article_type=v

Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
check_url/52203?article_type=v

Solid Phase Synthesis of a Functionalized Bis-Peptide Using “Safety Catch” Methodology
check_url/52203?article_type=v

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
check_url/52203?article_type=v

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Read Article