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Journal / Immunology and Infection / Sample Preparation of Mycobacterium tuberculosis…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Article DOI: 10.3791/3673-v • 07:56 min • September 3rd, 2012
September 3rd, 2012 •
1, 1, 2, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, 2Department of Chemistry, University of Nebraska-Lincoln

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The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

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Mycobacterium Tuberculosis Nuclear Magnetic Resonance Metabolomic Studies Sample Preparation Drug-resistant Strains Gene Function Gene Expression Analysis Global Patterns Metabolic Composition Small Molecular Weight Chemicals Target Identification Anti-tubercular Activity NMR Technology
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