A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:33Collection of Colonic Tissue Samples and Assessment of Colitis Severity
- 03:58Sample Preparation for Myeloperoxidase (MPO) Assay
- 05:09Quantification of Inflammatory Markers
- 06:01Evaluating the Severity of Acute Colitis in DSS-treated Animals
- 08:23Conclusion
Experimentella modeller för inflammatoriska tarmsjukdomar har tillåtit oss att undersöka de komplexa medfödda och adaptiva immunsvaret i samband med patogenes. Använda histologisk scoring, kvantifiering av proinflammatoriska cytokiner och myeloperoxidas aktivitet, kan man börja utvärdera dessa svar ses vid inflammatorisk tarmsjukdom.
Tags
Intestinal InflammationDSS-induced ModelIBDUlcerative ColitisCrohn’s DiseasePathogenesisGeneticEnvironmental ComponentsAnimal ModelsColonic InflammationDextran Sulphate SodiumColitis-inducing ChemicalsAcute ColitisChronic ColitisWeight LossLoose StoolDiarrheaRectal BleedingHistological AnalysisPro-inflammatory Cytokines
