Journal
/
Biology
/
Studere Mitotisk Checkpoint ved at illustrere Dynamic Kinetochore Protein Adfærd og kromosom Motion i levende<em> Drosophila</em> Syncytial Embryoner
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Studying Mitotic Checkpoint by Illustrating Dynamic Kinetochore Protein Behavior and Chromosome Motion in Living Drosophila Syncytial Embryos

Studere Mitotisk Checkpoint ved at illustrere Dynamic Kinetochore Protein Adfærd og kromosom Motion i levende<em> Drosophila</em> Syncytial Embryoner

DOI:
10.3791/3763-v

•

13:59 min

•

June 14, 2012

•

,
1Institute for Cell and Molecular Biosciences,University of Newcastle, United Kingdom

Chapters

  • 00:05Title
  • 01:45Maintaining Flies and Collecting Eggs
  • 03:43Preparing Coverslips and Slides
  • 05:10Dechorionating Embryos
  • 06:36Provoking the SAC by Microinjecting Embryos with Appropriate Reagents
  • 08:55Imaging Embryos
  • 10:08Results: Observing Dynamic Kinetochore Behavior and Chromosome Motion
  • 13:28Conclusion

Summary

Automatic Translation

Automatic Translation

Den kinetochore er, hvor SAC indleder sit signal overvåge mitotiske adskillelse af de søsterkromatider. En metode er beskrevet til at visualisere rekruttering og omsætning af en af ​​de kinetochore proteiner og sin samordning med kromosomet bevægelse i<em> Drosophila</em> Embryoer ved hjælp af et Leica laserscannings konfokalt system.

Tags

Mitotic CheckpointKinetochore ProteinChromosome MotionLiving Drosophila Syncytial EmbryosSAC MechanismAneuploidyGenomic InstabilityCancersBirth DefectsAlzheimer’sMad1Mad2Bub1BubR1Bub3Mps1Zw10RodAurora B KinaseCdc20APC/C ActivatorsCyclin BSecurinMetaphase To Anaphase TransitionSAC Signal InitiationKinetochore AssemblyRelay Onto APC/C InhibitionDrosophila Experimental System

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Studying Aggression in Drosophila (fruit flies)

Application of Light-cured Dental Adhesive Resin for Mounting Electrodes or Microdialysis Probes in Chronic Experiments

High-resolution Measurement of Odor-Driven Behavior in Drosophila Larvae

Microinjection Techniques for Studying Mitosis in the Drosophila melanogaster Syncytial Embryo

In-vivo Centrifugation of Drosophila Embryos

RNAi Interference by dsRNA Injection into Drosophila Embryos

Imaging Cell Shape Change in Living Drosophila Embryos

Identifikation af protein-protein interaktion i<em> Drosophila</em> Voksen Heads af Tandem Affinitetsrensning (TAP)

En In vivo Crosslinking tilgang til Isoler proteinkomplekser Fra Drosophila Embryoner

Genom-dækkende protein-protein-interaktion Screening af protein-fragmentet Komplementering Assay (PCA) i levende celler

Read Article