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Isolering af opløselige og uopløselige PrP Oligomerer i normal human hjerne
JoVE Journal
Medicine
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JoVE Journal Medicine
Isolation of Soluble and Insoluble PrP Oligomers in the Normal Human Brain
DOI:

11:29 min

October 03, 2012

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Chapters

  • 00:05Title
  • 02:02Preparation of Brain Homogenate and Detergent Soluble and Insoluble Fractions
  • 03:21Velocity Sedimentation in Sucrose Step Gradients
  • 04:30Size Exclusion Chromatography
  • 06:16Capture of PrP with g5P and Western Blotting
  • 08:20Representative PrP Isolation Results
  • 10:45Conclusion

Summary

Automatic Translation

En ny art af cellulær prionprotein (PrP<sup> C</sup>) Er for nylig blevet identificeret i uinficerede humane hjerner under anvendelse af fremgangsmåderne beskrevet her. Disse fremgangsmåder kan anvendes til at isolere forskellige PrP arter, mens nogle af dem er også anvendelige til isolering af andre fejlfoldede proteinaggregater fra humane hjerner.

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