Journal
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Immunology and Infection
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Encellede Analyse af Bacillus subtilis Biofilm Brug fluorescensmikroskopi og Flowcytometri
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Immunology and Infection
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JoVE Journal Immunology and Infection
Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Encellede Analyse af Bacillus subtilis Biofilm Brug fluorescensmikroskopi og Flowcytometri

DOI:
10.3791/3796-v

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13:28 min

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February 15, 2012

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, , ,
1Institute for Molecular Infection Biology (IMIB),University of Würzburg

Chapters

  • 00:05Title
  • 01:42Labeling B. subtilis and Biofilm Formation Assay
  • 05:30Biofilm Dispersion and Cell Fixation
  • 06:28Fluorescence Microscopy
  • 07:22Quantification of Single Cells Using Flow Cytometry
  • 08:47Results of B. subtilis Biofilm Analysis
  • 13:01Conclusion

Summary

Automatic Translation

Automatic Translation

Mikrobielle biofilm udgøres generelt af forskellige subpopulationer af specialiserede celler. Enkelt-celle-analyse af disse subpopulationer kræver anvendelse af fluorescerende reportere. Her beskriver vi en protokol til at visualisere og overvåge flere subpopulationswithin<em> B. subtilis</em> Biofilm med fluorescensmikroskopi og flow-cytometri.

Tags

Single-cell AnalysisBacillus SubtilisBiofilmsFluorescence MicroscopyFlow CytometryExtracellular MatrixProteinsExopolysaccharidesSpecialized CellsSpatial OrganizationTemporal OrganizationMatrix ProducersFluorescent ReportersSurfactin

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