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Chapters
- 00:05Title
- 01:15Culturing Mouse Embryonic Stem Cells (Timing: 3 days)
- 02:34Neural Induction (Timing: 2 days)
- 04:48Motor Neuron Specification (Timing: 5 days)
- 05:24Preparation of Poly-DL-Ornithine/Laminin Coated Coverslips (Timing: 2 days)
- 06:18Axon Elongation (Timing: 2 days)
- 07:47Results: mES Cell Differentiation into Motor Neurons
- 09:12Conclusion
Vi har udviklet en ny protokol for at forbedre effektiviteten af in vitro differentiering af muse embryonale stamceller i motoriske neuroner. De differentierede ES-celler opnået motoriske neuroner har som vist ved ekspressionen af neuronale og motorisk neuron markører under anvendelse immunohistokemiske teknikker.
Tags
Mouse Embryonic Stem CellsMotor NeuronsDifferentiationRetinoic AcidSonic HedgehogDisease MechanismsDrug CompoundsSpinal Muscular AtrophyAmyotrophic Lateral SclerosisProtocolsNeuralization ProcessNogginFibroblast Growth FactorsFGF SignalingNeurogenesisAnterior Neural PatterningPosterior Neural Identity
