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Metodo per l'isolamento e l'identificazione di mRNA, microRNA e componenti proteiche dei complessi Ribonucleoproteina da estratti cellulari con RIP-Chip
JoVE Journal
Biology
Author Produced
This content is Free Access.
JoVE Journal Biology
Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip
DOI:

13:34 min

September 29, 2012

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Chapters

  • 00:00Title
  • 01:43Harvesting RNP Complex
  • 03:59Preparation of Isolation Materials
  • 06:40Immunoprecipitation
  • 08:42DNase and Proteinase K Treatment and RNA Precipitation
  • 12:11Representative Results
  • 12:49Conclusions

Summary

Automatic Translation

Un passo dal protocollo passo per isolare e identificare complessi RNA associati attraverso RIP-Chip.

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