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实时定量PCR(qPCR的microRNA检测在前列腺肿瘤)
JoVE Journal
Medicine
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JoVE Journal Medicine
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
DOI:

08:30 min

May 16, 2012

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Chapters

  • 00:05Title
  • 01:51Prostate Sample Collection
  • 02:40Isolating Total RNA from Samples
  • 04:27Real-time PCR for Detection of miRNA
  • 06:10Analyzing the Data
  • 07:25Representative qPCR Results
  • 08:02Conclusion

Summary

Automatic Translation

实时定量聚合酶链反应(QPCR)是一种快速,灵敏的方法来探讨不同的microRNA(miRNA)的分子的表达水平在肿瘤样本。使用此方法数百种不同的miRNA分子表达可以被放大,量化,并从相同的cDNA模板分析。

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