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实时定量PCR（qPCR的microRNA检测在前列腺肿瘤）
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JoVE Journal Medicine
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR (qPCR)
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实时定量PCR（qPCR的microRNA检测在前列腺肿瘤）

DOI:
10.3791/3874-v

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08:30 min

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May 16, 2012

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, , , ,
1Department of Laboratory Medicine & Pathobiology,University of Toronto, 2Division of Urology,Sunnybrook Health Sciences Centre, Toronto, Canada, 3Department of Anatomic Pathology,Sunnybrook Health Sciences Centre, Toronto, Canada, 4Biological Sciences,Sunnybrook Research Institute

Chapters

  • 00:05Title
  • 01:51Prostate Sample Collection
  • 02:40Isolating Total RNA from Samples
  • 04:27Real-time PCR for Detection of miRNA
  • 06:10Analyzing the Data
  • 07:25Representative qPCR Results
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

实时定量聚合酶链反应（QPCR）是一种快速，灵敏的方法来探讨不同的microRNA（miRNA）的分子的表达水平在肿瘤样本。使用此方法数百种不同的miRNA分子表达可以被放大，量化，并从相同的cDNA模板分析。

Tags

MicroRNA DetectionProstate TumorsQuantitative Real-time PCR (qPCR)MiRNAsNon-coding RNA MoleculesCellular ProcessDevelopmentApoptosisCell Cycle RegulationGene Expression RegulationTarget Messenger RNAs (mRNAs)DysregulationBiomarkersTherapeutic AgentsTaqman Probe-based QPCRClinical SamplesAccuracyReproducibilitySpecificityWhole Genome AnalysesMicroarray StudiesRadical ProstatectomyClinical Variables

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