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亲和沉淀主动RHO上皮细胞裂解液中使用的GST标签的突变Rho蛋白(GST-RhoA蛋白(G17A))全环基金
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Affinity Precipitation of Active Rho-GEFs Using a GST-tagged Mutant Rho Protein (GST-RhoA(G17A)) from Epithelial Cell Lysates
DOI:

11:28 min

March 31, 2012

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Chapters

  • 00:05Title
  • 01:46Transformation of E. coli with the pGEX-RhoA(G17A) Construct
  • 02:55Preparation of GST-RhoA(G17A) Beads
  • 07:50GEF Pulldown Assay with Nucleotide-free RhoA(G17A) Beads
  • 10:11Representative Pull Down Results
  • 11:03Conclusion

Summary

Automatic Translation

这里介绍的方法检测,按照激活RhoA的具体GDP / GTP交换因子(全环基金)在培养细胞中的突变RhoA的GST融合蛋白具有高亲和力激活全环基金的使用。全环基金沉淀的细胞裂解物,免疫印迹和密度定量检测。

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