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Rotte mesenterium eksteriorisering: En model til at undersøge de cellulære Dynamics involveret i angiogenese
JoVE Journal
Biology
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JoVE Journal Biology
Rat Mesentery Exteriorization: A Model for Investigating the Cellular Dynamics Involved in Angiogenesis

Rotte mesenterium eksteriorisering: En model til at undersøge de cellulære Dynamics involveret i angiogenese

DOI:
10.3791/3954-v

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12:29 min

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May 20, 2012

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, , , ,
1Department of Biomedical Engineering,Tulane University, 2Department of Biomedical Engineering,University of Virginia , 3Center for Stem Cell Research and Regenerative Medicine,Tulane University

Chapters

  • 00:05Title
  • 02:06Rat Mesentary Exteriorization Model
  • 04:42Tissue Harvesting and Fixation
  • 06:06Tissue Immunolabeling: Colorimetric Labeling
  • 07:37Tissue Immunolabeling: Fluorescent Labeling
  • 08:27Results: Immunolabeling and Quantitative Analysis of Rat Mesentery Tissues
  • 11:35Conclusion

Summary

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Denne artikel beskriver en simpel model for at stimulere angiogenese hos rotter mesenterium. Model frembringer dramatiske stigninger i kapillær spire, vaskulær område og vaskulær densitet over en relativ kort tidsforløb i et væv, der tillader en face visualisering af hele mikrovaskulære net ned til en enkelt celle-niveau.

Tags

Rat Mesentery ExteriorizationCellular DynamicsAngiogenesisMicrovascular Network GrowthWound HealingInflammationDiabetic RetinopathyTumor GrowthArteriogenesisSpatial DynamicsTemporal DynamicsVessel Growth ManipulationReproducible ModelPro-angiogenic AgentsSurgical Intervention

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