JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Neuroscience
/
ラット胚神経細胞の分離と文化:クイックプロトコル
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Neuroscience
Isolation and Culture of Rat Embryonic Neural Cells: A Quick Protocol
Please note that all translations are automatically generated.
Click here for the English version.
ラット胚神経細胞の分離と文化:クイックプロトコル
DOI:
10.3791/3965-v
•
10:51 min
•
May 24, 2012
•
Marco Pacifici
2
,
Francesca Peruzzi
2
1
LSU Health Sciences Center – New Orleans
,
2
Medical School and Stanley S. Scott Cancer Center
Chapters
00:05
Title
01:42
Poly-D-Lysine: Preparation and Coating of the Plastic Cell Culture Dishes
03:03
PDL and Laminin: Preparation and Coating of the Glass Two-chamber Slides
04:07
Neuronal Dissection and Culture
09:40
Results: Isolation and Culture of Rat Embryonic Neural Cells
10:27
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
我々は、齧歯類の胚から海馬および皮質ニューロンを分離、培養するための迅速な方法論を記述します。このプロトコルは、私たちはほぼ純粋な神経細胞の培養が必要とされる実験を行うことができます。
Tags
Isolation
Culture
Rat Embryonic Neural Cells
Quick Protocol
Dissociation
Hippocampal Neurons
Cortical Neurons
E15-17 Rat Embryos
Mouse Primary Neurons
Human Primary Neurons
Neural Progenitors
Serum-free Medium
Nucleofection
Immunocytochemistry
Nucleic Acids Preparation
Electrophysiology
Lentiviral Transduction
Calcium Phosphate Transfection
Lipid-based Transfection
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
胚の脊髄から交連ニューロンの解剖と文化
ニューロスフェアのアッセイを使用して成体マウス神経幹細胞の単離と拡大
ニューロスフェアのアッセイを使用してマウス胎児神経幹細胞培養の確立
神経コロニー形成細胞アッセイ:神経前駆細胞から善意の神経幹細胞を区別するアッセイ
人間の毛包から神経堤幹細胞の単離および培養
マウス胚の運動ニューロンのレクチンに基づく分離と文化
胎児マウス神経管から神経堤細胞の単離および培養
の解離、文化、および分析<em>アフリカツメガエル</em>胚性網膜組織
胚前脳からの内皮細胞の単離および培養
マウスを一回、二つの文化:個々のマウスの二つの神経原性のゾーンから成体神経幹細胞の単離および培養
Read Article