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Solubilization and Bio-conjugation of Quantum Dots and Bacterial Toxicity Assays by Growth Curve and Plate Count
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Chapters
- 00:05Title
- 01:37QD Solubilization
- 03:32QD Conjugation to Antibiotic
- 05:40Determination of Antibiotic IC50 and QD Toxicity
- 08:18Assessing Antibiotic-conjugated QD Toxicity
- 08:58Assessing QD Toxicity by Plate Count
- 10:01Representative Assay Results
- 12:34Conclusion
Nanoparticles such as semiconductor quantum dots (QDs) can be used to create photoactivatable agents for anti-microbial or anti-cancer applications. This technique shows how to water-solubilize cadmium telluride (CdTe) QDs, conjugate them to an antibiotic, and perform a bacterial inhibition assay based upon growth curves and plate count.
Tags
Quantum DotsSolubilizationBio-conjugationBacterial Toxicity AssaysGrowth CurvePlate CountFluorescenceImagingDiagnosticsTherapyCytotoxic AgentsTargeted Killing Of BacteriaMultiply-resistant Bacterial StrainsPublic Health CrisisGram Negative PathogensNanomaterialsAntimicrobial EffectToxicity Of Nanoparticles To BacteriaSemiconductor NanoparticlesTiO2ZnOCuOOxidationReactive Oxygen Species (ROS)Metal Ions
