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Oct4GiP Reporter Assay auf Gene, die Maus embryonalen Stammzellen Wartungs-und Self-Study Erneuerung Regulate
JoVE Journal
Biology
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JoVE Journal Biology
Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
DOI:

08:01 min

May 30, 2012

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Chapters

  • 00:05Title
  • 01:38siRNA Transfection in Oct4GiP Cells
  • 04:05FACS Analysis
  • 06:07Results: The Oct4GiP Reporter Assay Readily Detects the Differentiation Caused by Gene Silencing in the Maintenance of ES Cell Renewal
  • 07:30Conclusion

Summary

Automatic Translation

Wir beschreiben einen Fluoreszenz-Reporter-Assay schnell zu identifizieren und zu charakterisieren, Gene, die Maus embryonalen Stammzellen Wartungs-und Selbst-Erneuerung zu regulieren.

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