A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 01:38siRNA Transfection in Oct4GiP Cells
- 04:05FACS Analysis
- 06:07Results: The Oct4GiP Reporter Assay Readily Detects the Differentiation Caused by Gene Silencing in the Maintenance of ES Cell Renewal
- 07:30Conclusion
Tags
Oct4GiPReporter AssayGenesRegulateMouse Embryonic Stem Cell MaintenanceSelf-renewalPluripotencyMolecular MechanismDevelopmental Biology StudiesDisease ModelingDrug DiscoveryRegenerative MedicineFluorescence Reporter-based AssaySelf-renewal StatusMouse ES CellsOct4 Gene Promoter RegionGreen Fluorescent Protein (GFP)DifferentiationFluorescence-activated Cell Sorting (FACS) AnalysisRNAiConvenienceSensitivityQuantitative AnalysisCost-effective
