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Fluorescence in situ hybridization (FISH) Protocol in Human Sperm
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Fluorescence in situ hybridization (FISH) Protocol in Human Sperm

Article DOI: 10.3791/1405 • 16:20 min • September 1st, 2009
September 1st, 2009 •
1, 1
1Unitat de Biologia Cel·lular (Facultat de Biociències), Universitat Autónoma de Barcelona

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Summary
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This video-article describes, step by step, how to process a semen sample to achieve good-quality fluorescence in situ hybridization on human spermatozoa. Preparations obtained can be used for aneuploidy screening in the context of clinical diagnosis.

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Fluorescence In Situ Hybridization FISH Protocol Human Sperm Aneuploidies Chromosomal Abnormalities Meiotic Errors Gametogenic Process Numerical Chromosome Abnormalities Offspring Clinical Diagnosis Genetic Reproductive Advice Chromosomes X Y 13 18 21 Semen Sample Processing Sperm Chromatin Decondensation And Denaturation Sperm FISH Preparations Microscope Visualization
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