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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Article DOI: 10.3791/4002-v 10:16 min May 5th, 2012
May 5th, 2012



A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.


Viral RNA Fluorescence In Situ Hybridization FISH Viral Replication Host Cell Function Viral Gene Products Membrane Organelles Mobile Vesicles Molecular Motor Proteins Intracellular Trafficking RNA Localization Gene Expression Cascades Obligate Parasites
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