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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)
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Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

Detection of Viral RNA by Fluorescence in situ Hybridization (FISH)

DOI:
10.3791/4002-v

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10:16 min

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May 05, 2012

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, ,
1Lady Davis Institute for Medical Research,Sir Mortimer B. Davis Jewish General Hospital, 2Department of Microbiology and Immunology,McGill University , 3Department of Medicine, Division of Experimental Medicine,McGill University

Chapters

  • 00:05Title
  • 01:11Cell Fixation
  • 03:15Fluorescence In situ Hybridization (FISH)
  • 05:11Immunofluorescence Staining
  • 07:07Results: Localization of HIV-1 RNA and Cellular Proteins in Transfected Cells
  • 09:34Conclusion

Summary

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A fluorescence in situ hybridization (FISH) method was developed to visually detect viral genomic RNA using fluorescence microscopy. A probe is made with specificity to the viral RNA that can then be identified using a combination of hybridization and immunofluorescence techniques. This technique offers the advantage of identifying the localization of the viral RNA or DNA at steady-state, providing information on the control of intracellular virus trafficking events.

Tags

Viral RNAFluorescence In Situ HybridizationFISHViral ReplicationHost Cell FunctionViral Gene ProductsMembrane OrganellesMobile VesiclesMolecular Motor ProteinsIntracellular TraffickingRNA LocalizationGene Expression CascadesObligate Parasites

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