Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Sujata S. Jha; Anton A. Komar

doi:10.3791/4026

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Isolering af ribo Bundet Gryende polypeptider In vitro Til at identificere Translationelle Pause steder langs mRNA

Isolation of Ribosome Bound Nascent Polypeptides <em>in vitro</em> to Identify Translational Pause Sites Along mRNA

JoVE Journal
Biology

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JoVE Journal Biology

Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Isolering af ribo Bundet Gryende polypeptider In vitro Til at identificere Translationelle Pause steder langs mRNA

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16,237 Views

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10:15 min

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July 06, 2012

DOI:

10.3791/4026-v

DOI:

10.3791/4026-v

•

10:15 min

July 06, 2012

•

2 Views

Sujata S. Jha¹, Anton A. Komar¹

¹Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Summary

En teknik til at identificere translationelle pause-steder på mRNA, er beskrevet. Denne procedure er baseret på isolering af nascente polypeptider akkumuleres på ribosomer under in vitro-translation af et mål-mRNA, efterfulgt af størrelses-analyse af nascente kæder under anvendelse af en denaturerende gelelektroforese.