Journal
/
Biology
/
Isolering af ribo Bundet Gryende polypeptider<em> In vitro</em> Til at identificere Translationelle Pause steder langs mRNA
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA

Isolering af ribo Bundet Gryende polypeptider<em> In vitro</em> Til at identificere Translationelle Pause steder langs mRNA

DOI:
10.3791/4026-v

•

10:15 min

•

July 06, 2012

•

,
1Center for Gene Regulation in Health and Disease, Department of Biological, Geological and Environmental Sciences,Cleveland State University

Chapters

  • 00:05Title
  • 03:55DNA Template Preparation, In vitro Transcription, and In vitro Translation
  • 05:30Isolation and Resolution of Nascent Polypeptides
  • 06:54Representative Translational Pause Results
  • 08:35Conclusion

Summary

Automatic Translation

Automatic Translation

En teknik til at identificere translationelle pause-steder på mRNA, er beskrevet. Denne procedure er baseret på isolering af nascente polypeptider akkumuleres på ribosomer under in vitro-translation af et mål-mRNA, efterfulgt af størrelses-analyse af nascente kæder under anvendelse af en denaturerende gelelektroforese.

Tags

RibosomePolypeptidesTranslational Pause SitesMRNACodon UsageSynonymous CodonsNon-uniform ElongationMRNA Secondary StructureRibosome MovementTranslational Elongation RatesCodon BiasTRNAsRare CodonsPause SitesProtein ExpressionMRNA StabilityProtein Folding

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4026?article_type=v

PAR-CliP - A Method to Identify Transcriptome-wide the Binding Sites of RNA Binding Proteins
check_url/4026?article_type=v

In vitro Transskription og lukning af Gaussia luciferase-mRNA Efterfulgt af HeLa Celletransfektion
check_url/4026?article_type=v

Brug SecM Arrest Sequence som et redskab til at isolere ribo bundne polypeptider
check_url/4026?article_type=v

In vitro-syntese af modificeret mRNA for Induktion af protein-ekspression i humane celler
check_url/4026?article_type=v

Måling oxidativ stress Modstand af<em> Caenorhabditis elegans</em> I 96-brønds mikrotiterplader
check_url/4026?article_type=v

Undersøgelse af proteiner bundet til Nascent DNA i pattedyrceller Brug BrdU-chip-slot-vestlige Teknik
check_url/4026?article_type=v

Måling stivheden af<em> Ex vivo</em> Mus Aortas Brug Atomic force mikroskopi
check_url/4026?article_type=v

En protokol til brug af gen sæt berigelse analyse til at identificere den passende dyremodel for Translationel forskning
check_url/4026?article_type=v

In Vitro SUMOylation analyse at studere SUMO E3 Ligase aktivitet
check_url/4026?article_type=v

Hurtig In Vivo Fiksering og isolering af translationelle komplekser fra eukaryote celler

Read Article