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Biology

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Isolering af ribo Bundet Gryende polypeptider
 
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Isolering af ribo Bundet Gryende polypeptider

Article DOI: 10.3791/4026
July 6th, 2012

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Summary July 6th, 2012

Please note that all translations are automatically generated.

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En teknik til at identificere translationelle pause-steder på mRNA, er beskrevet. Denne procedure er baseret på isolering af nascente polypeptider akkumuleres på ribosomer under in vitro-translation af et mål-mRNA, efterfulgt af størrelses-analyse af nascente kæder under anvendelse af en denaturerende gelelektroforese.

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