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July 06, 2012
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Summary
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وصفت وهناك تقنية لتحديد المواقع وقفة متعدية على مرنا. ويستند هذا الإجراء على عزل البروتينية وليدة تراكم على ريبوسوم في المختبر خلال ترجمة لمرنا هدف، يليه تحليل حجم السلاسل الوليدة باستخدام هلام إستشراد يبدل طبيعة المكونات.
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Cite this Article
Jha, S. S., Komar, A. A. Isolation of Ribosome Bound Nascent Polypeptides in vitro to Identify Translational Pause Sites Along mRNA. J. Vis. Exp. (65), e4026, doi:10.3791/4026 (2012).
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