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流感病毒从感染细胞的染色质的核糖核蛋白复合物的亲和纯化
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Immunology and Infection
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JoVE Journal Immunology and Infection
Affinity Purification of Influenza Virus Ribonucleoprotein Complexes from the Chromatin of Infected Cells
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流感病毒从感染细胞的染色质的核糖核蛋白复合物的亲和纯化

DOI:
10.3791/4028-v

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11:20 min

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June 03, 2012

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,
1Department of Virology,Universitätsklinikum Freiburg

Chapters

  • 00:05Title
  • 02:12Subcellular Fractionation
  • 06:38Strep-tag Purification
  • 08:39Analysis of Purified Ribonucleoprotein Complexes
  • 10:38Conclusion

Summary

Automatic Translation

Automatic Translation

流感病毒与宿主细胞染色体复制他们在协会的RNA基因组。在这里，我们提出了一个方法来净化完整的病毒核蛋白复合物，从感染细胞的染色质。纯化病毒复合物，可以分析，Western blot和引物蛋白质和RNA含量的扩展，分别。

Tags

Affinity PurificationInfluenza VirusRibonucleoprotein ComplexesChromatinNegative-strand RNA VirusesViral GenomeNucleoproteinPolymerase ComplexViral RNA SynthesisViral MRNA SynthesisCellular Pre-mRNAsChromatin InteractionsHost RNA Polymerase IIHost NucleosomesRecombinant Influenza VirusesOne-Strep-TagPB2 SubunitAffinity Purification Of VRNPsLysis Procedures

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