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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
JoVE Journal
Medicine
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JoVE Journal Medicine
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers

DOI:
10.3791/4050-v

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12:24 min

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July 17, 2012

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, , , , , , , ,
1Thayer School of Engineering,Dartmouth College, 2Department of Physics and Astronomy,Dartmouth College, 3Darmouth Medical School,Dartmouth College, 4School of Computer Science,University of Birmingham

Chapters

  • 00:05Title
  • 02:51Animal Preparation
  • 04:53Fluorescence Tomography System Calibration
  • 06:15Imaging Protocol
  • 08:45Image Reconstruction
  • 10:34Results: Fluorescence Reconstruction
  • 11:06Conclusion

Summary

Automatic Translation

Automatic Translation

Diffuse fluorescence tomography offers a relatively low-cost and potentially high-throughout approach to preclinical in vivo tumor imaging. The methodology of optical data collection, calibration, and image reconstruction is presented for a computed tomography-guided non-contact time-domain system using fluorescent targeting of the tumor biomarker epidermal growth factor receptor in a mouse glioma model.

Tags

Computed Tomography-guided Time-domain Diffuse Fluorescence TomographySmall AnimalsCancer BiomarkersPreclinical Drug DiscoveryDevelopment StudiesLight AbsorptionOptical Signal PropagationVisible WavelengthsRed And Near-infrared (near-IR) Light AbsorptionNon-elastic ScatteringFluorescent AgentsImaging SystemsLight Propagation ModelsWhole Body Three-dimensional ImagingIll-posed Nature Of Diffuse Fluorescence TomographyStabilityContrast RecoverySpatial ResolutionImage Reconstruction TechniquesFMI In Small AnimalsCharge-coupled Device (CCD)-based SystemsSensitivity Detectors

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