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JoVE Journal Medicine
Kleuring Protocols for Human pancreatische eilandjes
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Kleuring Protocols for Human pancreatische eilandjes

Article DOI: 10.3791/4068
May 23rd, 2012 •
1, 1, 1, 1, 1
1Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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May 23rd, 2012

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Deze video demonstreert de procedures voor de karakterisering van humane pancreatische eilandjes met hematoxyline en eosine (H & E) en immunohistochemie (IHC). Pancreas delen van het hoofd, lichaam en staart regio's worden gekleurd door zowel H & E en IHC te eilandje endocriene samenstelling (insuline, glucagon, en pancreas polypeptide), cel-replicatie (Ki67) en inflammatoire infiltraten (H & E, CD3) te bepalen. De uncinate regio is gelokaliseerd met behulp van IHC voor pancreas polypeptide.

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