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基因转移到老年人的鸡胚<em>前OVO</em>电穿孔
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Gene Transfer into Older Chicken Embryos by ex ovo Electroporation
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基因转移到老年人的鸡胚<em>前OVO</em>电穿孔

DOI:
10.3791/4078-v

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06:10 min

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July 27, 2012

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, , ,
1Albrecht-Kossel-Institute for Neuroregeneration,School of Medicine University of Rostock, 2Institute of Anatomy I,School of Medicine University of Jena

Chapters

  • 00:05Title
  • 01:22Ex ovo Culture
  • 02:51Preparation for Ex ovo Electroporation
  • 03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
  • 04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

介绍将在以后的孵化阶段（比汉堡和汉密尔顿阶段（HH）22岁以上）的鸡胚胎的基因转移方法。这种方法克服缺点<em>在OVO</em>电适用于年龄较大的鸡胚胎是一种有用的技术，研究基因功能和调控，在旧的发展阶段。

Tags

Gene TransferChicken EmbryosEx Ovo ElectroporationGene FunctionGene RegulationIn VivoDNA PlasmidsSiRNA PlasmidsRNA OligosMorpholino Antisense OligonucleotidesIncubation StagesVitelline MembraneAllantoic VesselsGene Transfer Protocol

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