Journal
/
Biology
/
Gene Transfer i ældre kyllingeembryoer af<em> Ex ovo</em> Elektroporation
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Gene Transfer into Older Chicken Embryos by ex ovo Electroporation

Gene Transfer i ældre kyllingeembryoer af<em> Ex ovo</em> Elektroporation

DOI:
10.3791/4078-v

•

06:10 min

•

July 27, 2012

•

, , ,
1Albrecht-Kossel-Institute for Neuroregeneration,School of Medicine University of Rostock, 2Institute of Anatomy I,School of Medicine University of Jena

Chapters

  • 00:05Title
  • 01:22Ex ovo Culture
  • 02:51Preparation for Ex ovo Electroporation
  • 03:51Gene Transfer into Chicken Optic Tectum by Ex ovo Electroporation
  • 04:43Results: Overexpression of Cad7 and GFP by Ex ovo Electroporation
  • 05:50Conclusion

Summary

Automatic Translation

Automatic Translation

En fremgangsmåde til genoverførsel til kyllingeembryoner på senere inkubering trin (ældre end Hamburger og Hamilton trin (HH) 22) er beskrevet. Denne fremgangsmåde overvinder ulemperne<em> In ovo</em> Elektroporation anvendes til ældre kyllingeembryoer og er en nyttig teknik til at studere gen-funktion og regulering på ældre udviklingsstadier.

Tags

Gene TransferChicken EmbryosEx Ovo ElectroporationGene FunctionGene RegulationIn VivoDNA PlasmidsSiRNA PlasmidsRNA OligosMorpholino Antisense OligonucleotidesIncubation StagesVitelline MembraneAllantoic VesselsGene Transfer Protocol

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Nuclear Transfer into Mouse Oocytes

In Utero Intraventricular Injection and Electroporation of E15 Mouse Embryos

Placing Growth Factor-Coated Beads on Early Stage Chicken Embryos

In Ovo Electroporations of HH Stage 10 Chicken Embryos

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation

In utero and ex vivo Electroporation for Gene Expression in Mouse Retinal Ganglion Cells

Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency

Xenotransplantation of Human Stem Cells into the Chicken Embryo

An ex-ovo Chicken Embryo Culture System Suitable for Imaging and Microsurgery Applications

Read Article