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Oprettelse af Definerede Gasformige Miljøer at studere effekten af ​​hypoxi på<em> C. elegans</em
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Creating Defined Gaseous Environments to Study the Effects of Hypoxia on C. elegans

Oprettelse af Definerede Gasformige Miljøer at studere effekten af ​​hypoxi på<em> C. elegans</em

DOI:
10.3791/4088-v

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11:07 min

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July 20, 2012

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, ,
1Department of Biochemistry,University of Washington, 2Molecular and Cellular Biology Program,University of Washington

Chapters

  • 00:05Title
  • 01:27Construction of Environmental Chambers
  • 02:56Connecting the Gas Supply to the Environmental Chamber
  • 04:39Preparation of Samples for Viability Assay
  • 07:32Rapid Harvest of Hypoxia-exposed Worms
  • 10:11Results: The Effect of Hypoxia on C. elegans
  • 10:44Conclusion

Summary

Automatic Translation

Automatic Translation

Dette papir detaljer hvordan du bruger kontinuerlig-flow hypoxi kamre til at skabe atmosfære med definerede koncentrationer af O<sub> 2</sub> At forstå biologiske reaktioner på nedsat O<sub> 2</sub>. Dette system er let at installere og vedligeholde, og fleksibel nok til at passe en bred vifte af O<sub> 2</sub> Koncentrationer og modelsystemer

Tags

Defined Gaseous EnvironmentsHypoxiaC. ElegansCellular Signaling PathwaysTreatment StrategiesModel OrganismGenetically AmenableHypoxic ResponseO2 ConcentrationsTranscriptional ResponseHif-1Ppm O2

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