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一个简单的协议提取血细胞从野生毛毛虫
JoVE Journal
Biology
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JoVE Journal
Biology
A Simple Protocol for Extracting Hemocytes from Wild Caterpillars
Please note that all translations are automatically generated.
Click here for the English version.
一个简单的协议提取血细胞从野生毛毛虫
DOI:
10.3791/4173-v
•
07:35 min
•
November 15, 2012
•
Teresa M. Stoepler
,
Julio C. Castillo
,
John T. Lill
,
Ioannis Eleftherianos
1
Department of Biological Sciences
,
The George Washington University
Chapters
00:05
Title
02:15
Injection of Caterpillars with Collection Solution
04:06
Extraction of Hemocytes
05:18
Quantification of Hemocytes
06:32
Representative Results
06:48
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
昆虫的血细胞进行了许多重要的功能,包括免疫和非免疫,昆虫生长发育的各个阶段的。我们目前的知识来自血细胞类型和功能对抗虫基因模型的研究。在这里,我们提出了野生毛毛虫血细胞提取,量化和可视化的方法。
Tags
Hemocytes
Wild Caterpillars
Insect Immune Response
Lymph Gland
Embryonic Hemocytes
Larval Stages
Insect Innate Immune Response
Cell Migration
Chemotaxis Regulation
Cell Apoptosis
Embryogenesis
Cell Spreading
Cell Aggregation
Nodules Formation
Phagocytosis
Encapsulation
Antimicrobial Peptides
Effector Molecules
Hemocyte Morphology
Hemocyte Function
Non-model Insect Species
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