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Chapters
- 00:05Title
- 02:33BioBrick Assembly
- 03:37Alkane Converstion Resting Cell Assay, in vivo
- 04:46Alkane Conversion Enzyme Assay, in vitro
- 06:18Ethyl Acetate Hydrocarbon Extraction and Concentration Measurements
- 07:40Alcohol/Aldehyde Dehydrogenase Activity Assay
- 08:49pCaiF Characterization and Tolerance Assay
- 10:26Homolog Interaction Mapping
- 11:11Representative Hydrocarbon Conversion Results
- 19:46Conclusion
En bæredygtig auto regulering bakterielt system til rensning af olie forureninger blev konstrueret ved hjælp af standard-udskiftelige DNA dele (BioBricks). Et konstrueret<em> E. coli</em> Stamme blev anvendt til at nedbryde alkaner via β-oxidation i toksiske vandige miljøer. De respektive enzymer fra forskellige arter viste alkan nedbrydningsaktivitet. Derudover en øget tolerance over<em> N</em>-Hexan blev opnået ved at indføre gener fra alkan-modstandsdygtige bakterier.
Tags
Hydrocarbon ConversionAqueous EnvironmentsEscherichia ColiToolkitAlkanesBioBricksGene Expression RegulationSurvival In Toxic EnvironmentsAlkane Degradation PathwayAlkanolsAlkanalsAlkanoic-acidsβ-oxidation PathwayMedium-chain AlkanesCycloalkanesAlkane Hydroxylase SystemGordonia Sp. TF68Long-chain AlkanesGeobacillus ThermodenitrificansADHALDHEnzyme ActivityGlucose ConditionsSubstrate-regulated Promoter
