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鞘糖脂抗原的质谱分析
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Mass Spectrometric Analysis of Glycosphingolipid Antigens
DOI:

13:09 min

April 16, 2013

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Chapters

  • 00:05Title
  • 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
  • 02:50Separation of Neutral and Acidic Lipids
  • 05:03Removal of Phospholipids from the Neutral Fraction
  • 08:14Permethylation of Glycosphingolipids
  • 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
  • 12:12Conclusion

Summary

Automatic Translation

一个特异和敏感的方法来深入了解糖脂抗原的免疫器官和细胞的表达谱。该方法利用离子阱质谱可以逐步碎裂的糖脂分子结构分析,比较,化学​​合成的标准。

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