Journal
/
Immunology and Infection
/
鞘糖脂抗原的质谱分析
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Please note that all translations are automatically generated. Click here for the English version.

鞘糖脂抗原的质谱分析

DOI:
10.3791/4224-v

•

13:09 min

•

April 16, 2013

•

, ,
1Undergraduate Program,Rice University , 2Proteomics Facility, Department of Pathology,University of Texas MD Anderson Cancer Center , 3Department of Melanoma Medical Oncology,University of Texas MD Anderson Cancer Center , 4University of Texas Graduate School of Biological Sciences at Houston

Chapters

  • 00:05Title
  • 01:47Extraction of Glycosphingolipids from Rat Leukemia Cells
  • 02:50Separation of Neutral and Acidic Lipids
  • 05:03Removal of Phospholipids from the Neutral Fraction
  • 08:14Permethylation of Glycosphingolipids
  • 10:04Results: Mass Spectra of Permethylated Glycosphingolipids
  • 12:12Conclusion

Summary

Automatic Translation

Automatic Translation

一个特异和敏感的方法来深入了解糖脂抗原的免疫器官和细胞的表达谱。该方法利用离子阱质谱可以逐步碎裂的糖脂分子结构分析，比较，化学​​合成的标准。

Tags

Mass Spectrometric AnalysisGlycosphingolipid AntigensGSL’sGlycoconjugate BiomacromoleculesStructural InformationEmbryonic DevelopmentSignal TransductionImmune Receptor RecognitionSugar MoietiesIsomersLipid MoietiesFatty Acyl Chain LengthUnsaturationHydroxylationCarbohydrate PortionCeramide PortionTri-hexosylceramidesGlobotriaosylceramide (Galα4Galβ4Glcβ1Cer)Isoglobotriaosylceramide (Galα3Galβ4Glcβ1Cer)Molecular MassesSugar LinkagesBiological FunctionsModification Of Ceramide PartAlpha-galactosylceramideCytokine Secretion ProfilesInvariant NKT CellsCancer ModelsAutoimmune DiseasesStructural Analysis Of IsomersImmune Organs And Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

酶联免疫斑点法（ELISPOT）：TH - 1细胞对微生物抗原的免疫应答的定量

紧凑型超支化聚乙二醇接枝的膜抗原受保护的功能性红血细胞

抗原驱动结肠炎模型的开发由抗原呈递细胞T细胞抗原研究的介绍

利用X射线晶体学，生物物理学，和功能分析，以确定管理机制T细胞癌抗原的受体识别

纯化膜隔间跨演示文稿中的外源抗原内质网关联降解

非遗传表面修饰的素-生物素系统在牛分枝杆菌卡介苗中的外源抗原表达

一种新型大鼠自然杀伤细胞无饲养系统的研究体外

大鼠外周全血细胞免疫表型和因子生成的单细胞分析

作为酶和抗原的非重组显示系统的孢子吸附

通过转版免疫沉淀和质谱测定,在HIV-1复制过程中核子因子的识别

Read Article