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Ekstraktion af Tissue Antigens for Funktionelle analyser
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JoVE Journal Biology
Extraction of Tissue Antigens for Functional Assays

Ekstraktion af Tissue Antigens for Funktionelle analyser

DOI:
10.3791/4230-v

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08:32 min

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September 10, 2012

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, , ,
1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne

Chapters

  • 00:05Title
  • 01:13Preparing Spleen Tissue
  • 01:57Preparing Human Islets for Storage
  • 02:43Preparing Tissue Extract
  • 04:47Freeze Drying Extracts
  • 06:17Results: Functional T cell Assays of Extracts
  • 08:08Conclusion

Summary

Automatic Translation

Automatic Translation

En enkel protokol til fremstilling af ekstrakter af humane væv til anvendelse som en kilde til antigener i funktionelle T-celleassays er beskrevet. Denne fremgangsmåde tillader T-celle-reaktioner på vævs-afledte antigener, der skal måles<em> In vitro</em>.

Tags

Functional AssaysAdaptive Immune ResponseB CellsT CellsAutoimmune DiseasesCancerType 1 DiabetesProtein AntigensFunctional CompatibilityProtein ConcentrationLyophilizationImmune CellsCytokine ProductionProliferationPBMC

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