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使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡
JoVE Journal
Biology
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JoVE Journal Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
DOI:

12:44 min

October 11, 2012

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Chapters

  • 00:05Title
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Automatic Translation

我们提出了一个简单的协议,可视化地区的小鼠胚胎中的细胞程序性死亡(PCD)和鉴别胚胎干(ES)细胞培养,使用高度水溶性染料LysoTracker。

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