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使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡

JoVE Journal
Biology

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JoVE Journal Biology

Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

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使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡

DOI:

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12:44 min

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October 11, 2012

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Jennifer L. Fogel, Thu Zan Tun Thein, Francesca V. Mariani

¹Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Chapters

00:05Title
01:48LysoTracker Staining of Mouse Embryos
04:53LysoTracker Staining of Differentiating ES Cultures
06:51Mounting Samples for Imaging
07:44Viewing LysoTracker-stained Samples and Image Analysis
09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10Conclusion

Summary

Automatic Translation

我们提出了一个简单的协议，可视化地区的小鼠胚胎中的细胞程序性死亡（PCD）和鉴别胚胎干（ES）细胞培养，使用高度水溶性染料LysoTracker。

Tags

LysoTracker Programmed Cell Death PCD Embryos Differentiating Embryonic Stem Cells Apoptosis Caspase Enzyme Cascade Membrane Blebbing Nuclear Shrinking DNA Fragmentation Autophagy Immune Cells Non-professional Phagocytes Lysosomal Activity

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