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使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡
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Biology
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JoVE Journal
Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
Please note that all translations are automatically generated.
Click here for the English version.
使用LysoTracker检测及鉴别胚胎干细胞在胚胎细胞程序性死亡
DOI:
10.3791/4254-v
•
12:44 min
•
October 11, 2012
•
Jennifer L. Fogel
,
Thu Zan Tun Thein
,
Francesca V. Mariani
1
Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine
,
University of Southern California
Chapters
00:05
Title
01:48
LysoTracker Staining of Mouse Embryos
04:53
LysoTracker Staining of Differentiating ES Cultures
06:51
Mounting Samples for Imaging
07:44
Viewing LysoTracker-stained Samples and Image Analysis
09:21
LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
我们提出了一个简单的协议,可视化地区的小鼠胚胎中的细胞程序性死亡(PCD)和鉴别胚胎干(ES)细胞培养,使用高度水溶性染料LysoTracker。
Tags
LysoTracker
Programmed Cell Death
PCD
Embryos
Differentiating Embryonic Stem Cells
Apoptosis
Caspase Enzyme Cascade
Membrane Blebbing
Nuclear Shrinking
DNA Fragmentation
Autophagy
Immune Cells
Non-professional Phagocytes
Lysosomal Activity
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