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Brug af LysoTracker at opdage programmeret celledød i Embryoner og Differentiering embryonale stamceller
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JoVE Journal Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells

Brug af LysoTracker at opdage programmeret celledød i Embryoner og Differentiering embryonale stamceller

DOI:
10.3791/4254-v

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12:44 min

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October 11, 2012

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, ,
1Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine,University of Southern California

Chapters

  • 00:05Title
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Automatic Translation

Automatic Translation

Vi præsenterer en enkel protokol til at visualisere regioner af programmeret celledød (PCD) i museembryoer og skelne embryostamceller (ES) cellekulturer under anvendelse af en meget opløseligt farvestof kaldet LysoTracker.

Tags

LysoTrackerProgrammed Cell DeathPCDEmbryosDifferentiating Embryonic Stem CellsApoptosisCaspase Enzyme CascadeMembrane BlebbingNuclear ShrinkingDNA FragmentationAutophagyImmune CellsNon-professional PhagocytesLysosomal Activity

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