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El uso de LysoTracker para detectar la muerte celular programada en embriones y la diferenciación de células madre embrionarias
JoVE Journal
Biology
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JoVE Journal Biology
Use of LysoTracker to Detect Programmed Cell Death in Embryos and Differentiating Embryonic Stem Cells
DOI:

12:44 min

October 11, 2012

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Chapters

  • 00:05Title
  • 01:48LysoTracker Staining of Mouse Embryos
  • 04:53LysoTracker Staining of Differentiating ES Cultures
  • 06:51Mounting Samples for Imaging
  • 07:44Viewing LysoTracker-stained Samples and Image Analysis
  • 09:21LysoTracker Staining in Shh Null Embryos and Cultured Cells
  • 12:10Conclusion

Summary

Automatic Translation

Se presenta un protocolo simple de visualizar regiones de la muerte celular programada (PCD) en embriones de ratón y diferenciadores madre embrionarias (ES) de cultivos de células utilizando un colorante altamente soluble llamada LysoTracker.

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