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来自祖，人脑少突胶质细胞培养系统
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Neuroscience
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JoVE Journal Neuroscience
Progenitor-derived Oligodendrocyte Culture System from Human Fetal Brain
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来自祖，人脑少突胶质细胞培养系统

DOI:
10.3791/4274-v

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14:26 min

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December 20, 2012

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, , , , ,
1Laboratory of Molecular Medicine and Neuroscience,National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Laboratory of Neurophysiology,National Institute of Neurological Disorders and Stroke, National Institutes of Health

Chapters

  • 00:05Title
  • 02:20Initiating Differentiation of Neural Progenitor Cells into Progenitor-derived Oligodendrocytes
  • 05:45Flow Cytometry Assay
  • 09:29Immunofluorescence Microscopy
  • 11:22Representative Results
  • 13:54Conclusion

Summary

Automatic Translation

Automatic Translation

小学，人类胎儿脑源性，多能祖细胞增殖<em在体外</em同时保持分化成神经元和星形胶质细胞的能力。这项工作表明，通过调节选择生长因子，神经前体细胞可以被诱导分化阶段的少突胶质谱系。

Tags

Progenitor-derived Oligodendrocyte Culture SystemHuman Fetal BrainDifferentiationNeural ProgenitorsNeuronal Cell TypesGlial Cell TypesMolecular RegulationNeural Cell Lineage DevelopmentIn Vitro ExpansionGlial ProgenitorsSub-cortical White MatterCulture ConditionsMyelin Producing OligodendrocytesGalactocerebroside+O4+ Oligodendrocyte Precursor Or Progenitor Cells (OPC)Neural Precursor CellsSupport Cells (astrocytes And Neurons)Culture SystemIn Vitro ExperimentationPrimary Human OligodendrocytesPathogenesisNeurotropic Infectious Agents (human PolyomavirusJCV)Demyelinating DiseasesCentral Nervous System (CNS)

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