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JoVE Journal
Biology

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CRISPR / CAS系统内切酶的底物生成
 
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CRISPR / CAS系统内切酶的底物生成

Article DOI: 10.3791/4277
September 8th, 2012

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Summary September 8th, 2012

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Click here for the English version.

CRISPR / CAS系统介导的适应性免疫中的细菌和古细菌。提出了许多CA蛋白作为核糖核酸内切酶作用于不同长度的crRNA前体。在这里,我们说明了三种不同的方法,CAS核酸内切酶活性的生化分析以产生前crRNA的底物。

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