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JoVE Journal
Biology

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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法
 
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优化细胞分裂监测细胞示踪染料的染色和扩散建模方法

Article doi: 10.3791/4287
December 13th, 2012

Summary December 13th, 2012

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成功地利用监测免疫细胞的功能和增殖的细胞追踪染料涉及的几个关键步骤。我们描述的方法为:1)获得明亮,均匀,重现性好标签的膜染料; 2)选择荧光染料和数据采集条件; 3)选择一个模型来量化细胞增殖的基础上染料稀释。

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