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Chapters
- 00:05Title
- 02:19General Membrane Labeling with PKH26 Cell Tracking Dye
- 05:43Flow Cytometer Setup and Staining Verification
- 07:34Proliferation Model Selection for Cell Division Monitoring by Dye Dilution
- 10:16Results: Optimization of Cell Tracking Dye Staining for Monitoring Cell Division
- 22:03Conclusion
Succesfuld brug af cellesporing farvestoffer til at overvåge immun celle funktion og proliferation involverer flere kritiske trin. Vi beskriver metoder til: 1) opnåelse af lys, ensartet og reproducerbar etiket-ning med membran farvestoffer, 2) udvælgelse af fluorokromer og dataopsamling betingelser, og 3) at vælge en model til at kvantificere celleproliferation baseret på farvestof fortynding.
Tags
Optimized StainingProliferation ModelingCell Division MonitoringCell Tracking DyesFlow CytometryImage CytometryStem CellsProgenitor CellsMembrane TransferPrecursor Cell ProliferationImmune Regulatory CellsEffector Cell FunctionCommercially Available DyesMembrane DyesProtein DyesMulticolor StudiesOptimal Use Of Dyes
