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全基因组基因缺失<em>链球菌血统</em通过高通量PCR
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Biology
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JoVE Journal
Biology
Genome-wide Gene Deletions in
Streptococcus sanguinis
by High Throughput PCR
Please note that all translations are automatically generated.
Click here for the English version.
全基因组基因缺失<em>链球菌血统</em通过高通量PCR
DOI:
10.3791/4356-v
•
14:07 min
•
November 23, 2012
•
Xiuchun Ge
,
Ping Xu
1
The Philips Institute of Oral and Craniofacial Molecular Biology
,
Virginia Commonwealth University
Chapters
00:05
Title
01:24
High-throughput PCR Amplification and Purification
06:24
Preparation of Competent Cells, Cell Transformation and Antibiotic Selection
08:30
Mutant Confirmation and Storage
10:34
Results: Creation of Recombinant PCR Constructs and Correct Mutants
13:48
Conclusion
Summary
Automatic Translation
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Automatic Translation
已经建立一个高效的全基因组单基因突变的方法使用<em>链球菌血统</em作为一种模式生物。通过这种方法已经取得了高通量重组PCR和转换。
Tags
Genome-wide Gene Deletions
Streptococcus Sanguinis
High Throughput PCR
Transposon Mutagenesis
Single-gene Deletion
Bacteria
Gene Knockout
Mutant Library
Partial Gene Inactivation
High-throughput Technique
Model Organism
Gene Deletion Construct
Kanamycin Resistance Protein
Primers
PCR Amplification
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