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全基因组基因缺失<em>链球菌血统</em通过高通量PCR
JoVE Journal
Biology
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JoVE Journal Biology
Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
DOI:

14:07 min

November 23, 2012

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Chapters

  • 00:05Title
  • 01:24High-throughput PCR Amplification and Purification
  • 06:24Preparation of Competent Cells, Cell Transformation and Antibiotic Selection
  • 08:30Mutant Confirmation and Storage
  • 10:34Results: Creation of Recombinant PCR Constructs and Correct Mutants
  • 13:48Conclusion

Summary

Automatic Translation

已经建立一个高效的全基因组单基因突变的方法使用<em>链球菌血统</em作为一种模式生物。通过这种方法已经取得了高通量重组PCR和转换。

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