Isolating Primary Melanocyte-like Cells from the Mouse Heart

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Biology

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Biology

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JoVE Journal Biology

Isolating Primary Melanocyte-like Cells from the Mouse Heart

DOI:

10.3791/4357-v

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07:38 min

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September 29, 2014

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Hayoung Hwang, Fang Liu, Mark D. Levin, Vickas V. Patel

¹Penn Cardiovascular Institute, Perelman School of Medicine,University of Pennsylvania

Chapters

00:05Title
01:31Preparation
02:43Isolating Hearts from Neonatal Mouse Pups
03:09Enzymatic Digestion
04:25Plating Cardiac Melanocyte-like Cells
05:24Isolating Cardiac Melanocyte-like Cells from Adult Mouse Heart
06:25Results: Isolated Cardiac Melanocytes
07:07Conclusion

Summary

Automatic Translation

In this protocol, we identified a novel population of melanocyte-like cells (also known as cardiac melanocytes) in the hearts of mice and humans that contribute to atrial arrhythmia triggers in mice.

Isolating Primary Melanocyte-like Cells from the Mouse Heart

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